Sequencing:Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .. After 72 hours of incubation, the amoebae were detached from culture flask and harvested by centrifugation at 1000 g for10 minutes followed by three steps of washing using Page’s modified Neff’s Amoeba Saline medium (2 mM NaCl, 16 μM MgSO4, 27.2 μM CaCl2, 1 mM Na2HPO4, 1 mM KH2PO4).After 72 hours of incubation, the amoebae were detached from culture flask and harvested by centrifugation at 1000 g for10 minutes followed by three steps of washing using Page’s modified Neff’s Amoeba Saline medium (2 mM NaCl, 16 μM MgSO4, 27.2 μM CaCl2, 1 mM Na2HPO4, 1 mM KH2PO4).
Comparison:Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .. After 72 hours of incubation, the amoebae were detached from culture flask and harvested by centrifugation at 1000 g for10 minutes followed by three steps of washing using Page’s modified Neff’s Amoeba Saline medium (2 mM NaCl, 16 μM MgSO4, 27.2 μM CaCl2, 1 mM Na2HPO4, 1 mM KH2PO4).After 72 hours of incubation, the amoebae were detached from culture flask and harvested by centrifugation at 1000 g for10 minutes followed by three steps of washing using Page’s modified Neff’s Amoeba Saline medium (2 mM NaCl, 16 μM MgSO4, 27.2 μM CaCl2, 1 mM Na2HPO4, 1 mM KH2PO4).
Construct:Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .. After 72 hours of incubation, the amoebae were detached from culture flask and harvested by centrifugation at 1000 g for10 minutes followed by three steps of washing using Page’s modified Neff’s Amoeba Saline medium (2 mM NaCl, 16 μM MgSO4, 27.2 μM CaCl2, 1 mM Na2HPO4, 1 mM KH2PO4).After 72 hours of incubation, the amoebae were detached from culture flask and harvested by centrifugation at 1000 g for10 minutes followed by three steps of washing using Page’s modified Neff’s Amoeba Saline medium (2 mM NaCl, 16 μM MgSO4, 27.2 μM CaCl2, 1 mM Na2HPO4, 1 mM KH2PO4).
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